Copy RNA into cDNA so it can be amplified or sequenced.
Sub-techniques
RNA
Expression measurement
4 techniques4 virtual labs
0 of 4 practised
RT-qPCRCt · ΔΔCt
Quantify expression of specific genes relative to a reference gene.
Sub-techniques
Northern blotband size
Check the size and abundance of a specific transcript.
Expression microarrayssignal intensity
Measure thousands of transcripts by hybridization; largely replaced by RNA-seq.