Branch
RNA
5 groups12 techniques12 virtual labs
0 of 12 practised
- Isolation & QC2 techniques
- RNA extractionIsolate total RNA while keeping RNases out.
- RNA integrity checkConfirm RNA is intact before reverse transcription or sequencing.
0 of 2 practisedOpen Isolation & QC → - Expression measurement4 techniques
- Reverse transcriptionCopy RNA into cDNA so it can be amplified or sequenced.
- RT-qPCRQuantify expression of specific genes relative to a reference gene.
- Northern blotCheck the size and abundance of a specific transcript.
- Expression microarraysMeasure thousands of transcripts by hybridization; largely replaced by RNA-seq.
0 of 4 practisedOpen Expression measurement → - Localization2 techniques
- In situ hybridizationLocalize transcripts inside fixed cells or tissue sections.
- Spatial transcriptomicsMeasure gene expression while keeping each transcript's position in the tissue.
0 of 2 practisedOpen Localization → - Perturbation & synthesis3 techniques
- RNA interferenceKnock down a gene's mRNA to study loss of function.
- Antisense oligonucleotidesDegrade a specific RNA or change how it is spliced.
- In vitro transcriptionMake RNA from a DNA template for probes, guide RNAs or mRNA.
0 of 3 practisedOpen Perturbation & synthesis → - RNA–protein interactions1 techniques0 of 1 practisedOpen RNA–protein interactions →