Immunoprecipitationpartners on blot
Pull a protein out of lysate with an antibody to find what binds it.
Protein
Interactions & function
5 techniques5 virtual labs
0 of 5 practised
Pull-down assaysbound partners
Use a tagged bait protein to capture interaction partners.
Sub-techniques
Proximity labellinglabelled neighbours
Tag proteins near a bait inside living cells for later identification.
Proximity ligation assayspots per cell
Visualize two proteins sitting within about 40 nm of each other in cells.
Enzyme activity assaysKm · Vmax
Measure how fast an enzyme converts its substrate.