Branch
Protein
5 groups20 techniques20 virtual labs
0 of 20 practised
- Extraction & quantification3 techniques
- Cell lysis & protein extractionBreak open cells and solubilize proteins without degrading them.
- Subcellular fractionationSeparate nuclei, cytoplasm, membranes or organelles before analysis.
- Protein quantificationMeasure total protein so samples are loaded equally.
0 of 3 practisedOpen Extraction & quantification → - Separation & detection5 techniques
- SDS-PAGESeparate denatured proteins by molecular weight.
- 2D gel electrophoresisSeparate proteins by isoelectric point, then by size.
- Western blotDetect a specific protein with antibodies and compare its level across samples.
- ELISAQuantify a protein or antigen in a liquid sample such as serum or supernatant.
- Multiplex immunoassaysMeasure many cytokines or proteins in one small sample.
0 of 5 practisedOpen Separation & detection → - Production & purification4 techniques
- Recombinant expressionProduce a protein in a host organism from an expression construct.
- Affinity purificationCapture a tagged protein on a matching resin.
- Chromatography polishingSeparate proteins further by size, charge or hydrophobicity.
- Antibody productionGenerate antibodies against a target antigen.
0 of 4 practisedOpen Production & purification → - Interactions & function5 techniques
- ImmunoprecipitationPull a protein out of lysate with an antibody to find what binds it.
- Pull-down assaysUse a tagged bait protein to capture interaction partners.
- Proximity labellingTag proteins near a bait inside living cells for later identification.
- Proximity ligation assayVisualize two proteins sitting within about 40 nm of each other in cells.
- Enzyme activity assaysMeasure how fast an enzyme converts its substrate.
0 of 5 practisedOpen Interactions & function → - Proteomics3 techniques
- Mass spectrometry proteomicsIdentify and quantify proteins across a sample.
- Post-translational modification analysisMap phosphorylation, glycosylation and other modifications.
- Edman degradationRead a protein's N-terminal sequence one residue at a time.
0 of 3 practisedOpen Proteomics →