Branch
Sequencing
7 groups16 techniques16 virtual labs
0 of 16 practised
- Low-throughput1 techniques0 of 1 practisedOpen Low-throughput →
- Library preparation2 techniques
- Library constructionFragment, adapter-tag and index DNA or cDNA for a sequencer.
- Library QC & poolingCheck size and concentration, then pool libraries at equal molarity.
0 of 2 practisedOpen Library preparation → - Platforms2 techniques
- Short-read sequencingSequence millions of short fragments in parallel for most genomic assays.
- Long-read sequencingRead through repeats, structural variants and full-length transcripts.
0 of 2 practisedOpen Platforms → - DNA applications4 techniques
- Whole-genome sequencingCall variants across the whole genome.
- Exome & targeted panelsSequence only protein-coding regions or chosen genes.
- Amplicon & microbiome sequencingDeep-sequence targeted regions or profile a microbial community.
- Methylation sequencingMap DNA methylation base by base.
0 of 4 practisedOpen DNA applications → - RNA applications3 techniques
- RNA-seqProfile the whole transcriptome to find differentially expressed genes.
- Single-cell RNA-seqMeasure expression cell by cell to identify cell types and states.
- Specialized RNA sequencingResolve full-length isoforms, translation or RNA modifications.
0 of 3 practisedOpen RNA applications → - Epigenomics & regulation3 techniques
- Protein–DNA binding mapsMap where a protein or histone mark sits on the genome.
- ATAC-seqMap open, accessible chromatin.
- 3D genome mappingMap which genome regions touch each other in the nucleus.
0 of 3 practisedOpen Epigenomics & regulation → - Data analysis (dry lab)1 techniques0 of 1 practisedOpen Data analysis (dry lab) →