BenchMasteryMolecular biology techniques & virtual lab
Advanced path · 5 steps

Knock out a gene

Edit a gene with CRISPR, get the edit into cells and confirm it worked.

0 of 5 steps practisedStart with step 1: CRISPR-Cas9 editing →
  1. CRISPR-Cas9 editingNot started

    Design guides and choose how to deliver Cas9 to cut the gene.

    LearnOpen lab
  2. TransfectionNot started

    Get the editing components into the cells.

    LearnOpen lab
  3. GenotypingNot started

    Find out which cells carry the edit, and whether one copy or both are changed.

    LearnOpen lab
  4. Western blotNot started

    Confirm the protein is actually gone, not just the DNA changed.

    LearnOpen lab
  5. Stable cell line generationNot started

    Grow a clonal line so every later experiment uses the same edited cells.

    LearnOpen lab