Core path · 5 steps
Detect and measure a protein
Get protein out of cells, measure it, separate it by size and detect the one you care about.
0 of 5 steps practisedStart with step 1: Cell lysis & protein extraction →
- Cell lysis & protein extractionNot started
Break the cells open while keeping the protein, and its modifications, intact.
- Protein quantificationNot started
Measure total protein so every lane or well gets the same amount.
- Western blotNot started
Transfer the gel to a membrane and detect the target with an antibody.