Core path · 8 steps
Clone a gene
Copy a gene, put it into a plasmid, get it into bacteria and confirm the sequence: the classic cloning workflow.
0 of 8 steps practisedStart with step 1: PCR →
- Agarose gel electrophoresisNot started
Check that the PCR gave one band of the right size before you go further.
- Ligation-based cloningNot started
Join the insert into the vector at the right insert to vector ratio.
- Bacterial transformationNot started
Get the plasmid into competent E. coli and select the cells that took it up.
- DNA quantification & QCNot started
Measure how much plasmid you have and how pure it is before sequencing.
- Sanger sequencingNot started
Read the insert base by base to confirm there are no mutations.